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dbco  (MedChemExpress)
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The exosome release from MSC‐UCNPs in situ. (A) Verification of the biocompatibility of Ac4ManNAz with MSCs ( n = 3), **** p < 0.0001. (B) The covalent binding of <t>CY5‐DBCO</t> to nitroso‐labeled MSCs in vitro. (C–E) PET‐CT imaging enabled the detection of local exosome release in vivo. (F) Statistical analysis of 68 Ga‐based brain tracking signals released <t>by</t> <t>exosomes</t> ( n = 3), * p < 0.05, ** p < 0.01. (G) Statistical analysis of 68 Ga signals in the heart/blood pool ( n = 3).
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The exosome release from MSC‐UCNPs in situ. (A) Verification of the biocompatibility of Ac4ManNAz with MSCs ( n = 3), **** p < 0.0001. (B) The covalent binding of <t>CY5‐DBCO</t> to nitroso‐labeled MSCs in vitro. (C–E) PET‐CT imaging enabled the detection of local exosome release in vivo. (F) Statistical analysis of 68 Ga‐based brain tracking signals released <t>by</t> <t>exosomes</t> ( n = 3), * p < 0.05, ** p < 0.01. (G) Statistical analysis of 68 Ga signals in the heart/blood pool ( n = 3).
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The exosome release from MSC‐UCNPs in situ. (A) Verification of the biocompatibility of Ac4ManNAz with MSCs ( n = 3), **** p < 0.0001. (B) The covalent binding of <t>CY5‐DBCO</t> to nitroso‐labeled MSCs in vitro. (C–E) PET‐CT imaging enabled the detection of local exosome release in vivo. (F) Statistical analysis of 68 Ga‐based brain tracking signals released <t>by</t> <t>exosomes</t> ( n = 3), * p < 0.05, ** p < 0.01. (G) Statistical analysis of 68 Ga signals in the heart/blood pool ( n = 3).
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The exosome release from MSC‐UCNPs in situ. (A) Verification of the biocompatibility of Ac4ManNAz with MSCs ( n = 3), **** p < 0.0001. (B) The covalent binding of <t>CY5‐DBCO</t> to nitroso‐labeled MSCs in vitro. (C–E) PET‐CT imaging enabled the detection of local exosome release in vivo. (F) Statistical analysis of 68 Ga‐based brain tracking signals released <t>by</t> <t>exosomes</t> ( n = 3), * p < 0.05, ** p < 0.01. (G) Statistical analysis of 68 Ga signals in the heart/blood pool ( n = 3).
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dbco peg4 nhs ester  (Click Chemistry Tools)
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The exosome release from MSC‐UCNPs in situ. (A) Verification of the biocompatibility of Ac4ManNAz with MSCs ( n = 3), **** p < 0.0001. (B) The covalent binding of <t>CY5‐DBCO</t> to nitroso‐labeled MSCs in vitro. (C–E) PET‐CT imaging enabled the detection of local exosome release in vivo. (F) Statistical analysis of 68 Ga‐based brain tracking signals released <t>by</t> <t>exosomes</t> ( n = 3), * p < 0.05, ** p < 0.01. (G) Statistical analysis of 68 Ga signals in the heart/blood pool ( n = 3).
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The exosome release from MSC‐UCNPs in situ. (A) Verification of the biocompatibility of Ac4ManNAz with MSCs ( n = 3), **** p < 0.0001. (B) The covalent binding of CY5‐DBCO to nitroso‐labeled MSCs in vitro. (C–E) PET‐CT imaging enabled the detection of local exosome release in vivo. (F) Statistical analysis of 68 Ga‐based brain tracking signals released by exosomes ( n = 3), * p < 0.05, ** p < 0.01. (G) Statistical analysis of 68 Ga signals in the heart/blood pool ( n = 3).

Journal: Advanced Science

Article Title: Light‐Switched Mesenchymal Stem Cells for In Situ Exosome Amplification in Craniofacial Bone Defect Reconstruction

doi: 10.1002/advs.75519

Figure Lengend Snippet: The exosome release from MSC‐UCNPs in situ. (A) Verification of the biocompatibility of Ac4ManNAz with MSCs ( n = 3), **** p < 0.0001. (B) The covalent binding of CY5‐DBCO to nitroso‐labeled MSCs in vitro. (C–E) PET‐CT imaging enabled the detection of local exosome release in vivo. (F) Statistical analysis of 68 Ga‐based brain tracking signals released by exosomes ( n = 3), * p < 0.05, ** p < 0.01. (G) Statistical analysis of 68 Ga signals in the heart/blood pool ( n = 3).

Article Snippet: Upon release from the hydrogels, these exosomes were immediately bound by DBCO (HY‐42973, MedChemExpress) conjugated radionuclide 68 Ga, enabling their tracking.

Techniques: In Situ, Binding Assay, Labeling, In Vitro, Positron Emission Tomography-Computed Tomography, Imaging, In Vivo